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采用敞口山楂组培新梢研究糖和无机氮源对不定根形成的影响,含有高水平蔗糖的培养基比低水平蔗糖的培养基新梢生根率高。新梢对糖的这种反应与糖代谢和物理渗透都有关,用甘露醇作为渗透代替剂在低浓度蔗糖存在下无作用,当蔗糖浓度达到一定程度时(86.64mmol·1~(-1))能影响新梢的生根率。在培养基中分别加入NO_3~(-)-N或NH_4~(+)-N作为唯一氮源,或是在MS培养基中加入NO_3~-/NH_4~+比率为标准的混合氮源,在测试范围(0～120mmol·1~(-1))内均降低新梢的生根率,三者对生根的抑制作用大小顺序依次为NH_4~+>NO_3~(-)-NH_4~(+)-N>NO_3~(-)-N.NO_3~-/NH_4~+的比率影响生根。NO_3~-/NH_4~+比率与生根之间呈直线正相关。糖/氮比值高有利于生根。插穗中碳、氮的绝对量及碳/氮比率可作为山楂新梢生根的指标。
The effects of sugar and inorganic nitrogen on the formation of adventitious roots were studied by using shoots of hawthorn in vitro. The rooting rate of shoots with high sucrose was higher than that with low sucrose. The response of shoots to sugar was related to sugar metabolism and physical infiltration. Mannitol as infiltration substitute did not play a role in the presence of low concentration of sucrose. When sucrose concentration reached a certain level (86.64 mmol · 1 -1) ) Can affect the rooting rate of shoots. In the medium, NO_3 ~ (-) - N or NH_4 ~ (+) - N was added as the only nitrogen source or the mixed NO_3 ~ - / NH_4 ~ The rooting rate of shoots was reduced in the range of 0-120 mmol · -1 -1, the order of inhibiting effect on rooting was NH_4 ~ +> NO_3 ~ (-) - NH_4 ~ (+) - N> NO_3 ~ (-) - N.NO_3 ~ - / NH_4 ~ + ratio affected rooting. There was a linear positive correlation between NO_3 ~ - / NH_4 ~ + ratio and rooting. Sugar / nitrogen ratio is conducive to rooting. Cuttings in the carbon, the absolute amount of nitrogen and carbon / nitrogen ratio can be used as an indicator of hawthorn shoots.