Use of a Multiplex RT-PCR Assay for Simultaneous Detection of the North American Genotype Porcine Re

• 作 者: CHEN Hong-ying WEI Zhan-yong Z
• 机 构: CollegeofAnimalHusbandryandVeterinary
• 出 处: 中国农业科学：英文版
• 发表日期: 2010年7期
• 关键词: 猪繁殖与呼吸综合征病毒 PCR检测方法 日本脑炎病毒 猪流感病毒 同时检测 基因型 多重 RT Japanese encephalitis virus mul
• 基金项目: Acknowledgements This work was supported by a grant from the Out- standing Person Innovation Foundation of Henan, China （0621002100）.

A multiplex reverse transcriptase-polymerase chain reaction(multiplex RT-PCR) assay was developed and subsequently evaluated for its efficacy in the detection of multiple viral infections simultaneously,in swine.Specific primers for each of the 3 RNA viru