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目的通过对宫颈癌患者人巨细胞病毒(HCMV)临床分离株基因序列遗传变异分析,了解其与宫颈癌之间的相关性,从而为宫颈癌发病机制研究提供理论依据。方法从宫颈癌患者的细胞标本中分离HCMV,对其基因进行全长序列PCR扩增,并进行同源性比较。结果 PCR扩增HCMV基因全长序列分别为568、347、1 472、466和732bp;测序分析5个基因的突变均由碱基替换所引起,并且UL83基因第18、231位氨基酸的突变位点都是具有诱导CTL抗原决定簇反应能力的表位区,而UL32,UL44,UL137和UL145基因序列具有高度保守性,初步推测UL83基因CTL表位突变情况可能与宫颈癌患者HCMV感染有关联;同源性比较显示,HCMV基因与HCMV AD169株、Merlin株、Towne株同源性为97%~100%。结论 UL83基因的突变可能是导致HCMV感染的主要原因,且其第18、231位氨基酸的突变位点都是具有诱导CTL抗原决定簇反应能力的表位区,因此认为UL83基因CTL表位突变可能与宫颈癌患者HCMV感染有关联。
Objective To analyze the genetic variation of human cytomegalovirus (HCMV) clinical isolates from patients with cervical cancer and to find out the correlation between them and cervical cancer so as to provide a theoretical basis for the study of the pathogenesis of cervical cancer. Methods HCMV was isolated from the cell samples of patients with cervical cancer and the full-length sequence of the gene was amplified by PCR. The homology was compared. Results The full-length sequences of HCMV gene were 568, 347, 472, 466 and 732 bp, respectively. Sequencing analysis showed that the five mutations were all caused by base substitutions, and the mutation sites of amino acids 18 and 231 of UL83 gene Are all epitopes that have the ability to induce CTL epitopes. However, the sequences of UL32, UL44, UL137 and UL145 are highly conserved. It is preliminarily presumed that the mutation of CTL epitope of UL83 gene may be associated with HCMV infection in cervical cancer patients. The homology between HCMV gene and HCMV AD169 strain, Merlin strain and Towne strain was 97% -100%. Conclusion The mutation of UL83 gene may be the main cause of HCMV infection, and the mutation site of amino acid at position 18,231 is an epitope region capable of inducing CTL antigenic determinant response. Therefore, the mutation of UL83 gene CTL epitope may be considered And cervical cancer HCMV infection are associated.