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目的研究ＣＤＡⅡ、β肽、丹参、蛇毒（精制腹蛇抗栓酶）抗ＬＣＩＤ２０裸鼠人肝癌模型肿瘤早期切除术后复发及转移的疗效。方法以ＬＬＣＩＤ２０模型肿瘤接种裸鼠后１６天，在肿瘤转移发生前行肝癌切除，术后皮下分别注射以上４种制剂，对照组注射等体积的生理盐水，每组５只裸鼠，接种后３５天处死动物，观察动物体重、切缘复发瘤直径、肝内转移和远处转移情况。结果ＣＤＡＩ组、β肽组、丹参组、蛇毒组动物体重（ｇ）与对照组相比无显著差异（Ｐ＞００５）。切缘复发瘤直径（ｃｍ）分别为１１±０２、０５±０２（Ｐ＜００１）、０９±０６（Ｐ＞００５）、０６±０４（Ｐ＞００５）、０５±０３（Ｐ＜００１），肝内转移灶数目分别为７２±２３、２６±２３（Ｐ＜００１）、００±００（Ｐ＜００１）、２０±０７（Ｐ＜００１）、２０±１４（Ｐ＜００１），累及肝叶数分别为３９±０６、１８±１３（Ｐ＜００５）、００±００（Ｐ＜００１）、１６±０６（Ｐ＜００１）、１６±０９（Ｐ＜００１），肺内转移灶数（个／只）分别为２８±２５、００±００（Ｐ＜００５）、００±００?
Objective To investigate the effect of CDAII, βpeptide, Salvia miltiorrhiza, and snake venom (recombinant venom antithrombotic enzyme) on the recurrence and metastasis of LCID20 human hepatocellular carcinoma in nude mice. Methods 16 days after inoculation of nude mice with LLCID20 model tumors, hepatectomy was performed before tumor metastasis. The above four preparations were injected subcutaneously and the control group was injected with an equal volume of saline. Each group of 5 nude mice was inoculated. Animals were sacrificed 35 days later. Animal body weight, recurrence tumor diameter, intrahepatic metastasis, and distant metastasis were observed. Results There was no significant difference in body weight (g) between the CDAI group, β-peptide group, Salvia miltiorrhiza group, and snake venom group (P>005). The recurrence tumor diameter (cm) at the margin was 11±02, 05±02 (P<001), 09±06 (P>005), 0, respectively. 6±04 (P>005), 05±03 (P<001), the number of intrahepatic metastatic lesions were 72±23, 26±23, respectively. (P<001), 00±00 (P<001), 20±07 (P<001), 20±14 (P<0) 01) The number of hepatic involvements were 39±06, 18±13 (P<005), 00±00 (P<001), 16, respectively. ±06 (P<001), 16±09 (P<001), and the number of lung metastases (each/only) was 28±25, 00 respectively. ±00 (P<005), 00±00?