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１００份大肠癌组织，用ＲＴ、ＰＣＲ－ＳＳＳＣＰ检测ｐ５３基因ｃＤＮＡ突变；Ｐａｂ１８０１单抗免疫组化检测ｐ５３基及其蛋白高表达并对大肠癌术后病人作５年生存随访，比较上述二结果与大肠癌预后的关系。１００例大肠癌中，ＲＴ－ＰＣ、ＳＳＣＰ显示引例大肠癌ｐ５３基因ｃＤＮＡ突变（５１％）；Ｐａｂ１８０１阳性率６２％。ｐ５３基因ｃＤＮＡ突变和ｐ５３基因蛋白高表达与Ｄｕｋｅｓ分期无关，ｐ５３基因ｐＤＮＡ突变与ｐ５３基因蛋白高表达相关（Ｐ＜０．０５）。结果提示，Ｐａｂｌ８０１单抗检测ｐＪ３基因蛋白高表达直接相关（ｐ＜０．０５），ｐ５３基因ｃＤＮＡ突变是大肠癌预后差的分子生物学标志物（ｐ＜０．０５）。ｐ５３基因蛋白产物受多种因素影响不能反映大肠癌的预后。
In 100 colorectal cancer tissues, p53 gene cDNA mutations were detected by RT and PCR-SSSCP. The expression of p53 and its protein were detected by immunohistochemistry with Pab1801 monoclonal antibody. The 5-year survival of patients with colorectal cancer was followed up. The relationship between the prognosis of colorectal cancer. In 100 cases of colorectal cancer, RT-PC and SSCP showed a mutation in the cDNA of p53 gene in colorectal cancer (51%); the positive rate of Pab1801 was 62%. The mutation of p53 gene and the high expression of p53 protein were not related to the Dukes stage. The p53 gene mutation was related to the high expression of p53 protein (P<0.05). The results suggest that Pabl801 monoclonal antibody is directly related to the high expression of pJ3 gene protein (p<0.05). p53 gene mutation is a poor molecular biological marker of colorectal cancer (p<0.05). The p53 gene protein product is affected by many factors and does not reflect the prognosis of colorectal cancer.