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醋酸纤维薄膜电泳可将血清糖蛋白分成a_1,a_2,β和γ区带。已经有许多报告指出,癌患者血清中的糖蛋白增加。作者改良了电泳条件,增加了血清糖蛋白在醋酸纤维薄膜电泳中的分离程度,并发现未处理过的45%的腺癌病人及进展性的80%的腺癌病人呈现二条α_2糖蛋白区带,而其它类型的癌症或对照组仅呈现一条α_2糖蛋白区带。电泳在0.05M三羟甲基氨基甲烷-巴比妥缓冲液(pH8.9)中进行,醋酸纤维薄膜用Helena实验室TitanⅡ型。血清用量5微升,电泳27分钟,电压
Acetate membrane electrophoresis separates serum glycoproteins into a_1, a_2, β and γ bands. There have been many reports that glycoproteins in the serum of cancer patients have increased. The authors modified the electrophoresis conditions to increase the degree of separation of serum glycoproteins in acetate thin film electrophoresis and found that two untreated adenocarcinoma patients and 80% of patients with advanced adenocarcinoma showed two α 2 glycoprotein bands , While other types of cancer or control group showed only one α 2 glycoprotein band. Electrophoresis was carried out in 0.05M Tris-barbiturate buffer (pH 8.9) and cellulose acetate membranes were coated with Helena lab Titan type II. 5 microliters serum, electrophoresis 27 minutes, voltage