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目的 研究牡蛎多糖抗衰老作用机制.方法 利用非标记定量蛋白质组方法分析牡蛎多糖抗衰老作用机制.将去除高丰度蛋白后的小鼠血清样本经酶解后进行LC-MS/MS分析,利用Maxquant软件(版本号1.3.0.5)查库,进行LFQ非标定量分析.结果 本研究共鉴定到4 624个肽段,541个蛋白质.将模型组与牡蛎多糖治疗组进行t-test分析,得到20个差异表达蛋白质.对在小鼠数据库中查找到的19个差异蛋白质进行分析,发现这些差异蛋白质参与15个生物过程、具有8类分子功能、存在于5个细胞组分中(Level 2).这些差异表达蛋白质中,peroxiredoxin-2 (AC:Q61171)和malate dehydrogenase(AC:P14152)的上调以及xanthine dehydrogenase/oxidase(AC:Q00519)的下调可以提高机体清除自由基的能力,降低NO数量.结论 牡蛎多糖是通过提高机体清除自由基的能力来发挥其抗衰老作用的.“,”Objective To investigate the anti-aging mechanism of polysaccharides from Crassostrea gigas.Methods Label-free quantitative proteomics was used to analyze the effects of the polysaccharides on anti-aging.The low-abundant proteins in serum in mice were digested with trypsin,followed by LC-MS/MS.The MS data were analyzed using Maxquant (ver 1.3.0.5) against database.Results The results of MS data showed 4624 peptides,and 541 proteins were identified.There were 20 differentially expressed proteins according to t-test.Among these differential expressed proteins,19 proteins were found in mice database.They covered 15 biological processes,8 molecular functions and 5 cellular components (level 2).Among the 19 proteins,the up-regulated peroxiredoxin-2(AC:Q61171)and malate dehydrogenase(AC:P14152) and down-regulated xanthine dehydrogenase/oxidase (AC:Q00519) could improve the ability to scavenge free radicals,and decrease NO.Conclusion The polysaccharides from C.gigas prevented aging by scavenging free radicals.