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目的采用介孔二氧化硅MCM-41包载人参皂苷Rg3纳米粒,改善其水溶性,以人肺癌细胞A549为模型,研究MCM-41载药后促进药物吸收的机制。方法以介孔二氧化硅纳米粒MCM-41为载体;通过吸附法装载人参皂苷Rg3;通过透射电镜和激光粒度仪考察MCM-41的形态结构及粒径大小;分别应用差示扫描量热法(DSC),粉末X射线衍射法(PXRD)和傅里叶红外光谱法(FTIR)对载药体系进行固体状态的表征;通过体外溶出实验考察其体外溶出度,并通过细胞毒性实验和细胞摄取实验探究载药体系对A549细胞的作用及抑制细胞的增殖机制。结果成功制备了介孔二氧化硅纳米粒MCM-41,体外溶出实验显示,MCM-41可以显著提高人参皂苷Rg3溶出速率,载药体系能够被A549细胞摄取并抑制细胞增殖。结论介孔二氧化硅MCM-41具有良好的增溶作用,负载人参皂苷Rg3的给药体系具有用于治疗人肺癌的潜力。
OBJECTIVE To investigate the mechanism of MCM-41 drug absorption after drug loading by using mesoporous silica MCM-41 to encapsulate ginsenoside Rg3 nanoparticles to improve its water solubility. Methods Mesoporous silica nanoparticles MCM-41 was used as carrier. Ginsenoside Rg3 was loaded by adsorption method. Morphology and size of MCM-41 were investigated by transmission electron microscopy and laser particle sizer. Differential scanning calorimetry (DSC), powder X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to characterize the solid state of the drug-loaded system. The dissolution in vitro was investigated by in vitro dissolution experiments. The cytotoxicity and cell uptake The experiment explored the effect of drug-loading system on A549 cells and the mechanism of inhibiting cell proliferation. Results Mesoporous silica nanoparticles MCM-41 were prepared successfully. In vitro dissolution experiments showed that MCM-41 can significantly improve the dissolution rate of ginsenoside Rg3, and the drug-loading system can be taken up by A549 cells and inhibited cell proliferation. Conclusion The mesoporous silica MCM-41 has a good solubilizing effect and the drug delivery system loaded with ginsenoside Rg3 has the potential for treating human lung cancer.