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用RAPD分析6个农艺性状不同的小麦─黑麦抗条锈病衍生系材料,建立与黑麦抗条锈病基因连锁的分子标记。结果表明,所用6个随机引物中,4个引物在6个衍生系中扩增出6个黑麦特有的产物,其大小约197~980bp,分别被标记为J17,J18a,J18b,J11a,J11b和D5。其中,J17、J11b和D5标记在6个衍生系中都存在,是与黑麦抗条锈病基因连锁的RAPD标记;J11a在4个衍生系中存在;J18a和J18b在3个衍生系中存在。表明这6个衍生系可能涉及3~6个黑麦片段的易位,是不同于1B/1R类型的一批新的小麦─黑麦异易位系。结果还表明,RAPD分析在小麦遗传背景中检测异源染色体片段转移,是一项有效的技术,有望成为早代选择异易位系的一个分子指标。
RAPD analysis of six agronomic traits of wheat-rye resistance to stripe rust derived material, the establishment of rye resistance to stripe rust resistance genes linked to molecular markers. The results showed that among the six random primers used, six primers specific to rye were amplified from 6 derived lines and their sizes ranged from 197 to 980 bp, and were identified as J17, J18a, J18b, J11a and J11b And D5. Among them, J17, J11b and D5 markers were present in all six derived lines, which were RAPD markers linked to rye stripe rust resistance genes; J11a was present in four derived lines; J18a and J18b were found in three derived lines. This indicates that these six derivatives may involve the translocation of 3 ~ 6 rye fragments, which is a new batch of wheat-rye heterotransferases different from 1B / 1R. The results also show that RAPD analysis of heterozygous chromosomal transfer in wheat genetic background is an effective technique and is expected to become a molecular marker for early generation selection of heterotrans plant.