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采用气相色谱法对具有不同烟碱转化能力白肋烟烟株不同叶位和叶点的烟碱转化率进行测定。结果表明,非转化株和高转化株不同叶位以及同一叶片不同叶点的烟碱转化率分布比较一致,变异性较小,非转化株各样品烟碱转化率均在2%以下,高转化株烟碱转化率均在85%以上,不表现烟碱转化的嵌合特征,取样部位对转化株鉴别效果无显著影响。低转化和中转化烟株,不同叶位烟叶和不同叶点间烟碱转化率存在较大的变异性,表现明显的嵌合特征,低转化株不同叶位烟叶烟碱转化率的变异范围为2.5%～4.8%,不同叶点的变异范围为2.6%～7.0%;中转化株不同叶位烟碱转化率的变异范围为2.51%～90.6%,不同叶点的变异范围为2.7%～66.9%。由于一部分叶片和叶点的烟碱转化率低于3%的现行转化株鉴别标准,取样部位会对转化株的鉴别效果产生影响,降低转化株鉴别标准十分必要。
The nicotine conversion rates of different leaf positions and leaf points of Burley tobacco with different nicotine conversion ability were determined by gas chromatography. The results showed that the nicotine conversion rates of non-transformed and high-transformed plants at different leaf positions and the same leaves were consistent and the variability was relatively low. The nicotine conversion of each non-transformed plant was below 2% The conversion rate of nicotine in all strains was more than 85%, which did not show the chimeric characteristics of nicotine conversion. The sampling site had no significant effect on the identification of transformants. The results showed that there was significant variability in nicotine conversion rate between tobacco leaves at different leaf positions and different leaf positions, showing obvious chimeric characteristics. The variation range of nicotine conversion rate at different leaf positions was 2.5% -4.8%, and the variation range of different leaf points was 2.6% -7.0%. The range of variation of nicotine conversion in different leaf loci ranged from 2.51% to 90.6%, and the variation range from 2.7% to 66.9 %. Because part of the leaf and leaf point nicotine conversion rate of less than 3% of the current transformant identification criteria, the sampling site will have an impact on the identification of transformants, reducing the identification criteria for transformants is necessary.