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目的检测维吾尔族、汉族冠状动脉性心脏病(冠心病)患者动脉粥样硬化斑块中的特异性牙周致病菌-放线菌。方法选择2009-10-2011-05在新疆医科大学第一附属医院心外科行冠状动脉搭桥手术的患者,术中通过动脉内膜切除术收集冠状动脉粥样硬化斑块157例,维吾尔族88例,汉族69例,其中男性119例,女性38例,年龄(58.2±8.1)岁。采用Chelex-100法提取动脉粥样硬化斑块中的放线菌DNA,通过聚合酶链反应(PCR)检测斑块中的放线菌。结果 157例动脉粥样硬化斑块中检出放线菌23例,其中维吾尔族21例,汉族2例。细菌的PCR产物经测序,与GenBank数据库中的序列比对同源性达99%。结论动脉粥样硬化斑块中特异性牙周致病菌放线菌在维吾尔族、汉族中的检出率存在差异。
Objective To detect specific periodontal pathogens - actinomycetes in atherosclerotic plaques of Uygur and Han patients with coronary heart disease (CHD). Method Selection 2009-10-2011-05 In the First Affiliated Hospital of Xinjiang Medical University Cardiac Surgery in patients undergoing coronary artery bypass surgery, intra-arterial endarterectomy surgery to collect atherosclerotic plaque in 157 cases, 88 cases of Uygur , 69 Han patients, including 119 males and 38 females, with an average age of (58.2 ± 8.1) years. The actinomycete DNA in atherosclerotic plaque was extracted by Chelex-100 method and the actinomycetes in plaque was detected by polymerase chain reaction (PCR). Results 157 cases of atherosclerotic plaque detected actinomycetes 23 cases, of which 21 cases of Uighur, Han 2 cases. The bacterial PCR products were sequenced and their homologies to the sequences in the GenBank database were 99%. Conclusion The prevalence of actinomycetes in atherosclerotic plaque in Uygur and Han nationality is different.