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目的探索阿萨希毛孢子菌细胞外分泌酶的种类与活性。方法采用卵黄琼脂培养基、BSA-琼脂培养基和AIP-ZYM试剂盒,观察11株阿萨希毛孢子菌细胞外分泌酶的种类及酶活性。结果 11株阿萨希毛孢子菌中9株磷脂酶活性较强,2株酶活性弱;均未测得酸性蛋白酶分泌。AIP-ZYM试剂盒显示11株菌有共性分泌酶,也有部分菌株有特异性分泌酶。结论阿萨希毛孢子菌细胞外分泌酶种类多样,不同来源菌株产酶种类及酶活性亦不相同。
Objective To explore the types and activities of extracellular secretase from Aspergillus acitrensis. Methods The yolk agar medium, BSA-agar medium and AIP-ZYM kit were used to observe the types and enzyme activities of extracellular secretase in 11 Aspergillus acitreas. Results Among the 11 Aspergillus acitreas, 9 strains had strong phospholipase activity and 2 strains showed weak activity. No acid protease was detected. AIP-ZYM kit showed 11 strains of common secretase, some also have specific secretase. Conclusion There are many kinds of extracellular secretase enzymes in C. albicans aspergillus sp., And the strains and enzyme activities of different origin strains are also different.