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目的探讨HPV高危亚型感染病例同时做HPVL1壳蛋白检测的表达情况,为早期发现宫颈上皮内病变提供理论依据。方法对58例HPV高危亚型感染的病例同时进行HPVL1蛋白检测以及宫颈活检。结果HPVL1蛋白阳性为45例,阳性表达率77.6%(45/58),其中HPVL1蛋白阳性病例有43例诊断为慢性子宫颈炎,2例诊断为宫颈上皮内瘤变Ⅰ级(CINⅠ级)。HPVL1蛋白阴性病例有9例诊断为宫颈上皮内瘤变Ⅰ级(CINⅠ级),4例诊断为宫颈上皮内瘤变Ⅱ级(CINⅡ级)。结论明确有HPV高危亚型感染的病例HPVL1蛋白阴性的病例宫颈病变较重,HPV原位导流杂交检测与HPVL1蛋白检测联合应用有助于早期发现宫颈癌。
Objective To investigate the expression of HPVL1 capsid protein in high risk HPV subtypes and to provide a theoretical basis for early detection of cervical intraepithelial lesions. Methods 58 cases of HPV infection in high-risk subtypes HPVL1 protein detection and cervical biopsy. Results The positive rate of HPV L1 protein in 45 cases was 77.6% (45/58), of which 43 cases were diagnosed as chronic cervicitis and 2 cases were diagnosed as grade Ⅰ cervical intraepithelial neoplasia (CIN Ⅰ). Nine cases of HPV L1 protein negative cases were diagnosed as grade Ⅰ cervical intraepithelial neoplasia (CIN Ⅰ grade), and 4 cases were diagnosed as grade Ⅱ cervical intraepithelial neoplasia (CIN Ⅱ grade). Conclusions It is clear that cases with HPV subtypes HPV infection have severe cervical lesion in cases with negative HPV L1 protein. In situ hybridization with HPV in situ hybridization and HPV L1 protein detection may be helpful for the early detection of cervical cancer.