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目的 :开展诱导异位骨髓形成的研究 ,探讨骨髓形成及其调控机理。方法 :采用异种 (人 )脱钙骨基质粉 ,植入 Spraque-Dawley大鼠腹壁两侧皮下 ,建立异种脱钙骨基质粉诱导异位骨髓形成的动物实验模型。结果 :在诱导第 7天可见间充质细胞包绕并迁入骨基质性植入物的间隙 ,分化成原始软骨细胞、形成软骨组织。诱导第 13天 ,新生软骨组织进一步增加 ,成骨细胞、破骨细胞和血管位于其中 ,呈现软骨化骨现象 ,类骨样基质产生 ,成骨细胞下陷变为骨细胞。诱导第 17天 ,大多数软骨细胞集团内部腔隙融合并逐渐扩大形成初级骨髓组织。至 3 4天 ,原植入的脱钙骨基质大部分已被吸收并出现典型的骨髓组织 ,可见到大量髓细胞产生。在诱导的40、5 0、6 0天 ,诱导出的骨髓组织未见进一步增加 ,也未见明显萎缩。结论 :人、鼠骨诱导活性因子 ( BMP)具同源性 ,无种的特异性 ;诱导骨髓伴随软骨内骨化过程而产生 ,与骨组织的生成、发育完全一致
OBJECTIVE: To study the induction of ectopic bone marrow formation and to explore the mechanism of bone marrow formation and regulation. Methods: Animal model of heterogenous bone decalcified bone matrix powder induced by heterologous decalcified bone matrix powder was established by subcutaneous implantation of xenogenic (human) decalcified bone matrix powder into both sides of the abdominal wall of Spraque-Dawley rats. Results: On the 7th day of induction, mesenchymal cells were found to wrap around and migrate into the gap of bone matrix implants, differentiate into primitive chondrocytes and form cartilage tissue. On the 13th day of induction, the newborn cartilage tissue was further increased, in which osteoblasts, osteoclasts and blood vessels were located, which presented cartilaginous bone phenomenon, osteoid-like matrix and osteoblasts subsided into osteocytes. On the 17th day of induction, the majority of chondrocyte aggregates internal lamella fusion and gradually expanded to form primary myeloid tissue. By 34 days, most of the original implanted decalcified bone matrix had been absorbed and showed typical myeloid tissue, and a large number of myeloid cells could be seen. At 40, 50, 60 days after induction, there was no further increase in induced myeloid tissue and no significant atrophy. CONCLUSION: Human and mouse osteoinductive factor (BMP) are homologous and have no species specificity. Induced bone marrow is accompanied by endochondral ossification process, which is consistent with the formation and development of bone tissue