论文部分内容阅读
为探讨ｐ５３基因产物与卵巢恶性生殖细胞肿瘤的关系，采用ｐ５３基因产物的单克隆抗体，用免疫组化ＡＢＣ法对８２例卵巢恶性生殖细胞肿瘤和２０例正常卵巢组织的Ｐ５３基因蛋白表达进行了研究。结果显示：在正常卵巢组织中未见Ｐ５３蛋白表达，而在肿瘤中Ｐ５３蛋白表达率为２４．３９％，其阳性定位于胞核：在生殖细胞肿瘤不同组织学类型中表达程度不同，但其表达在各组织学类型间差异无显著性（Ｐ＞０．０５），而在临床分期中，Ⅲ、Ⅳ期卵巢生殖细胞肿瘤Ｐ５３蛋白表达阳性率明显高于Ⅰ、Ⅱ期（Ｐ＜０．０５），Ｐ５３蛋白表达阳性组患者死亡率明显高于阴性组Ｐ＜０．０１）。提示Ｐ５３蛋白表达可能与卵巢生殖细胞肿瘤恶性程度及预后有关
To investigate the relationship between p53 gene products and malignant ovarian germ cell tumors, p53 gene product monoclonal antibody was used to perform immunohistochemical ABC method on P53 gene protein expression in 82 cases of malignant ovarian germ cell tumors and 20 normal ovarian tissues. the study. The results showed that there was no expression of P53 protein in normal ovarian tissues, but the expression rate of P53 protein in tumors was 24.39%. The positive expression of P53 protein was located in the nucleus: the degree of expression was different in different histological types of germ cell tumors, but its There was no significant difference in expression between histological types (P>0.05). In clinical stages, the positive rate of P53 protein expression in stage III and IV ovarian germ cell tumors was significantly higher than that in stages I and II (P<0. 05) The mortality rate of patients with positive expression of P53 protein was significantly higher than that of negative patients (P<0.01). It is suggested that the expression of P53 protein may be related to malignancy and prognosis of ovarian germ cell tumors