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目的:探讨肥大细胞(MC)与血管活性肠肽(VIP)在肠易激综合征(IBS)患者结肠黏膜中的表达情况及二者的联系,为神经-内分泌-免疫网络分析IBS的发病机制提供一定理论依据。方法:选取腹泻型和便秘型IBS患者及正常对照者各20例,对其结肠黏膜活检标本进行免疫组化SABC法和甲苯胺蓝法染色后,对比观察MC和VIP表达情况。结果:IBS患者回盲部和乙状结肠部MC数和VIP免疫阳性神经纤维数均较对照组显著增多(P<0.01),而且IBS患者回盲部MC数和VIP免疫阳性神经纤维均较乙状结肠部MC数和VIP免疫阳性神经纤维明显增多。结论:MC和VIP可能参与IBS的病理生理过程。
OBJECTIVE: To investigate the expression of mast cells (MC) and vasoactive intestinal peptide (VIP) in colonic mucosa of patients with irritable bowel syndrome (IBS) and the relationship between them and to analyze the pathogenesis of IBS for neuroendocrine-immune network Provide a certain theoretical basis. Methods: Twenty patients with diarrhea-type and constipation-type IBS and normal controls were selected. Tissue mucosa biopsies were stained immunohistochemically with SABC and toluidine blue staining to compare the expression of MC and VIP. Results: The numbers of MC and VIP immunoreactive nerve fibers in ileocecal and sigmoid colon were significantly increased in patients with IBS (P <0.01), and the numbers of MCs and VIP immunoreactive nerve fibers in IBS were significantly higher than those in sigmoid colon Number and VIP immunoreactive nerve fibers increased significantly. Conclusion: MC and VIP may participate in the pathophysiology of IBS.