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AIM:To assess the three polymorphism regions withincytotoxic T-lymphocyte antigen 4(CTLA-4)gene,a C/Tbase exchange in the promoter region-318(CTLA-4-318C/T),an A/G substitution in the exon 1 position49(CTLA-4 49A/G),a T/C substitution in 1172(CTLA-4-1172T/C)in patients with chronic hepatitis B.METHODS:Fifty-one patients with chronic hepatitis Bvirus infection and 150 healthy subjects were recruitedsequentially as they presented to the hepatic clinic.Clas-sification of chronic hepatitis B virus(HBV)-infected pa-tients was as asymptomatic carrier state(26 patients)and chronic hepatitis B(25 patients).Genomic DNA wasisolated from anti-coagulated peripheral blood Buffy coatusing Miller’s salting-out method.The presence of theCTLA-4 gene polymorphisms was determined using poly-merase chain reaction amplification refractory mutationsystem(ARMS).RESULTS:We observed a significant association be-tween-318 genotypes frequency(T+C-,T+C+,T-C+)and susceptibility to chronic hepatitis B(P=0.012,OR=0.49,95%CI:0.206-1.162).However,we did notobserve a significant association for +49 genotype fre-quency(T+C+,T+C- T-C+)and -1172 genotype fre-quency(C+T+,T+C- C+T-)and state of disease.CONCLUSION:Our results suggest that CTLA-4 genepolymorphisms may partially be involved in the suscepti-bility to chronic hepatitis B.
AIM: To assess the three polymorphism regions within cytotoxic T-lymphocyte antigen 4 (CTLA-4) gene, a C / T base exchange in the promoter region-318 (CTLA- 4-318C / T), an A / G substitution in the exon 1 position49 (CTLA-4 49A / G), a T / C substitution in 1172 (CTLA-4-1172T / C) in patients with chronic hepatitis B. METHODS: Fifty-one patients with chronic hepatitis B virus infection and 150 healthy subjects were recruited sequentially as they presented to the hepatic clinic. Claas-sification of chronic hepatitis B virus (HBV) -infected pa-tients was as asymptomatic carrier state (26 patients) and chronic hepatitis B (25 patients). Genomic DNA wasisolated from anti-coagulated peripheral blood Buffy coat using Miller’s salting-out method. The presence of the CTLA-4 gene polymorphisms was determined using poly-merase chain reaction amplification refractory mutations system (ARMS) .RESULTS: We observed a significant association be- tween-318 genotypes frequency (T + C-, T + C +, T-C +) and susceptibility to chronic hepatitis B (P = 0.012, OR = 0). 49,95% CI: 0.206-1.162) .owever, we did not observe a significant association for +49 genotype fre-quency (T + C +, T + C- T-C +) and -1172 genotype fre- quency (C + T + , T + C-C + T-) and state of disease. CONCLUSION: Our results suggest that CTLA-4 genepolymorphisms may partially be involved in the susceptibility to chronic hepatitis B.