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有人认为,流行性出血热(简称流热)的免疫学发病机理和第Ⅲ型变态反应有关。某些实验室对本病患者血清中和组织中免疫复合物做了检查。国内外报道,采用免疫荧光法检查流热肾组织中免疫复合物,但荧光法需要荧光显微镜,标本也不易保存。而免疫酶组化法可以克服上述缺点,并且在观察沉积物时,清晰度和准确性都较好。用本法观察流热肾组织免疫复合物,国内刊物尚未见报导。为了进一步观察组织中的免疫复合物,探讨其发病机理,作者采用辣根过氧化物酶(HRP)标记抗体的组化法对病肾IgG 及C_3免疫复合物的沉积进行了观察。一、材料与方法1.流热尸体肾脏:取自1例33岁男性尸体肾脏。根据病理常规巨检和HE 染色切片镜检所见,并结合全国流热临床诊断标准确定为流热。
Some people think that the immunological pathogenesis of epidemic hemorrhagic fever (referred to as fluid heat) and type Ⅲ allergy related. Some laboratories have examined the immune complexes in serum and tissue in patients with this disease. Domestic and foreign reports, the use of immunofluorescence flow heat kidney tissue immune complexes, but the fluorescence method requires fluorescence microscopy, the specimen is not easy to save. The immunohistochemical method can overcome the above shortcomings, and in the observation of sediment, clarity and accuracy are better. The use of this method to observe the heat-flow immune kidney tissue immune complexes, domestic publications have not been reported. In order to further observe the immune complex in tissues and to explore its pathogenesis, the authors used the HRP-labeled antibody histochemical method to observe the deposition of pathological kidney IgG and C_3 immune complexes. First, the material and methods 1 fluid hot body kidney: taken from a 33-year-old male body kidney. According to histopathological macroscopic examination and HE staining section of the microscopic findings, combined with the clinical diagnostic criteria for heat flow to determine the heat flow.