论文部分内容阅读
目的了解早期肠内免疫营养对烫伤大鼠全身及肠道免疫功能的影响。方法将健康SD大鼠分为标准营养组(EN组)和免疫营养组(EIN组),每组32只。将两组大鼠制成烧伤总面积30%TBSA的Ⅲ度烫伤模型,于伤后1、4、7、10 d检测其外周血T淋巴细胞亚群、肠黏膜增殖细胞核抗原(PCNA)表达水平、浆细胞数量及肠黏液分泌型免疫球蛋白A(S-IgA)含量的变化。另取8只健康大鼠检测上述指标作为正常参考值。结果(1)与正常值比较,伤后EN组CD3~+、CD4~+、CD4~+、CD8~+降低但CD8~+升高,与各项指标大部分时相点比较,差异有统计学意义(P<0.05或P<0.01)。伤后10 d与EN组(CD4~+/CD8~+为1.26±0.10)比较,EIN组CD3~+、CD4~+、CD4~+/CD8~+(CD4~+/CD8~+为1.86±0.25)升高而CD8~+下降(P<0.01)。(2)伤后4、7、10 d,EIN组肠黏膜PCNA表达水平、浆细胞数量及肠黏液S-IgA含量较EN组明显升高(P<0.05或P<0.01)。结论烫伤后早期给予肠内免疫营养,可以提高全身及肠道免疫功能,效果优于标准肠内营养。
Objective To understand the effect of early enteral immunization on systemic and intestinal immune function in scalded rats. Methods Healthy SD rats were divided into standard nutrition group (EN group) and immunized nutrition group (EIN group), 32 in each group. The rats of the two groups were made into the third-degree scald model of 30% TBSA with burn, and the levels of T lymphocyte subsets and the expression of proliferating cell nuclear antigen (PCNA) in peripheral blood were detected at 1, 4, 7 and 10 days after injury , The number of plasma cells and the content of secreted immunoglobulin A (S-IgA) in intestinal mucosa. Another 8 healthy rats to detect the above indicators as a normal reference value. Results (1) Compared with normal group, the levels of CD3 ~ +, CD4 ~ +, CD4 ~ + and CD8 ~ + in EN group after injury were significantly higher than those in normal group Significance (P <0.05 or P <0.01). The levels of CD3 +, CD4 +, CD4 + / CD8 + (CD4 + / CD8 +) in EIN group were 1.86 ± 0.25) and decreased CD8 ~ + (P <0.01). (2) At 4, 7 and 10 days after injury, the expression of PCNA, the number of plasma cells and the content of S-IgA in intestinal mucosa in EIN group were significantly higher than those in EN group (P <0.05 or P <0.01). Conclusion The early post-scald immunization of enteral nutrition can improve the systemic and intestinal immune function, the effect is better than the standard enteral nutrition.