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应用纯培养菌制备标本，以生理盐水为空白对照，共检测１１７例，并试验了实际标本的处理方法。从ＰＣＲ法、菌培养、凝集试验入选判别模型的顺序、真实性评价及ＲＯＣ曲线面积大小比较３个诊断试验的判别能力的大小。结果表明，３个诊断试验均入选判别模型，它们对诊断霍乱的判别能力均显著，３个指标的入选顺序为：ＰＣＲ试验→菌培养→凝集试验，第一个入选的指标是Ｘ１（ＰＣＲ），其对判别函数的贡献最大，判别能力也最大，其次是菌培养，最后是凝集试验；以判别函数为标准，ＰＣＲ试验的灵敏度及特异度均可达到１００％，细菌培养灵敏度为１００％，特异度为９６．５５％，假阳性率为３．４５％；凝集试验灵敏度为６４．７７％，特异度为９６．５５％，假阳性率为３．４５％，假阴性率为３５．２３％，３种试验的真实性评价表明ＰＣＲ试验的真实性高于其他两个试验；３种试验的ＲＯＣ曲线面积由大到小顺序是：ＰＣＲ试验（０．９６１７）＞菌培养（０．９５９６）＞凝集试验（０．６３３４），故诊断霍乱的判别能力由大到小的顺序是：ＰＣＲ试验＞菌培养＞凝集试验。ＰＣＲ试验的判别能力大于菌培养、凝集试验
The pure culture bacteria were used to prepare the specimens, and saline was used as the blank control. A total of 117 specimens were tested and the actual specimens were tested. The order of the discriminant models, the authenticity evaluation and the size of the ROC curve area were compared by PCR method, bacterial culture and agglutination test to compare the discriminatory power of three diagnostic tests. The results showed that all the three diagnostic tests were included in the discriminant model. All of them were significant in discriminating the diagnosis of cholera. The order of the three indexes was PCR test → bacterial culture → agglutination test. The first selected index was X1 (PCR) , Which has the largest contribution to the discriminant function and has the highest discrimination ability, followed by the bacterial culture and finally the agglutination test. With the discriminant function as the standard, the sensitivity and specificity of the PCR test can reach 100%, the sensitivity of the bacterial culture is 100% The specificity was 96.55%, the false positive rate was 3.45%. The agglutination test was 64.77%, the specificity was 96.55%, the false positive rate was 3.45%, the false negative rate was 35.23 %. The authenticity evaluation of the three tests showed that the PCR test was more truthful than the other two tests. The order of the ROC curves of the three tests was: PCR test (0.9617)> bacterial culture (0.9596 )> Agglutination test (0.6334), so the diagnostic ability of cholera from large to small Shun They are: PCR Test> bacterial culture> agglutination test. PCR test discrimination ability than bacterial culture, agglutination test