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目的建立登革热实验室早期诊断方法。方法采用酶联免疫吸附试验(ELISA)检测登革病毒(DENV)NS1抗原,实时聚合酶链反应(Real-time PCR)检测DENV RNA并分型,对2014年广东省暴发流行期间疑似登革热的病例进行实验室早期诊断,并对两种检测方法进行比较分析。结果272例研究对象中267例临床诊断为登革热,DENV NS1抗原阳性245例,阳性率为90.1%(245/272);DENV RNA阳性256例,阳性率为94.1%(256/272)。256例核酸阳性病例中DENV-1型224例,占87.5%(224/256);DENV-2型15例,占5.9%(15/256);DENV-1型和DENV-2型同时阳性17例,占6.6%(17/256)。两种方法检出的符合率为93.0%(253/272)。结论2014年广东省登革热暴发流行主要以DENV-1为主,同时存在DENV-2型散在流行及少数DENV-1型和DENV-2型合并感染;ELISA方法检测DENV NS1抗原及Real-time PCR检测DENV RNA的两种检测方法,有助于登革热的早期检出。
Objective To establish an early diagnosis method of dengue fever laboratory. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect Dengue virus (NDV) NS1 antigen. The real-time polymerase chain reaction (Real-time PCR) was used to detect DENV RNA and genotyped the cases. Laboratory early diagnosis, and comparative analysis of the two detection methods. Results Of the 272 subjects, 267 were clinically diagnosed as dengue fever, 245 positive for DENV NS1 antigen (90.1%, 245/272), and 256 positive for DENV RNA (94.1%, 256/272). Among 256 cases of nucleic acid positive, 224 cases were DENV-1 type (87.5%), 15 cases were DENV-2 type (5.9%), 17 cases were positive for DENV-1 and DENV-2 Cases, accounting for 6.6% (17/256). The coincidence rate of the two methods was 93.0% (253/272). Conclusion The predominant epidemic of dengue fever in Guangdong Province in 2014 was mainly DENV-1, with concurrent epidemic of DENV-2 and a few cases of DENV-1 and DENV-2 infection. The detection of DENV NS1 antigen and Real-time PCR by ELISA Two methods of detection of DENV RNA contribute to the early detection of dengue fever.