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目的:观察曲古菌素A(Trichostatin A,TSA)对心肌缺血再灌注损伤大鼠心肌的保护作用。方法:Wistar大鼠,随机分为6组:假手术组;模型组;阳性药组;TSA 0.05,0.1,0.2 mg.kg-13个剂量组。采用结扎冠状动脉左前降支(LAD)的方法制备大鼠心肌缺血再灌注损伤(I/R)模型,手术前连续5 d ip给药,缺血30 min再灌注24 h后观察TSA对心肌I/R模型大鼠心肌梗死面积、组织病理学及血清中超氧化物歧化酶(SOD)、丙二醛(MDA)含量的影响。结果:与模型组相比,TSA能降低心肌I/R模型大鼠心肌梗死面积,增加血清SOD活性,减少脂质过氧化物MDA含量,对I/R引起的心肌组织病理结构改变有明显改善作用。结论:TSA对缺血再灌注损伤大鼠心肌具有保护作用。
Objective: To observe the protective effect of Trichostatin A (TSA) on myocardium of myocardial ischemia-reperfusion injury in rats. Methods: Wistar rats were randomly divided into 6 groups: sham operation group, model group, positive drug group, TSA 0.05,0.1,0.2 mg.kg-13 dose group. The rat model of myocardial ischemia-reperfusion (I / R) injury was established by ligation of left anterior descending coronary artery (LAD). The rats were dosed ip for 5 d before operation and reperfused for 24 h after ischemia. Effect of I / R model on myocardial infarct size, histopathology and serum superoxide dismutase (SOD) and malondialdehyde (MDA) contents. Results: Compared with the model group, TSA could reduce myocardial infarct size, increase serum SOD activity, decrease lipid peroxidation MDA content in myocardial I / R model rats, and significantly improve myocardial histopathological changes induced by I / R effect. Conclusion: TSA has a protective effect on myocardial ischemia-reperfusion injury in rats.