论文部分内容阅读
目的:研究黄花柳花总黄酮对在体心肌缺血再灌注损伤大鼠模型是否具有保护作用并探讨其相关机制。方法:结扎大鼠心脏左冠状动脉前将支30 min,造成缺血状态后再灌2 h,实时监测心电图ST段变化。采用腹主动脉采血,检测血清中LDH,CK-MB,AST,GSH-PX,SOD,MDA,MPO,IL-6和TNF-α等相关指标变化,利用苏木素-伊红(HE)染色观察心肌组织病理学改变。结果:与模型组比较,给药组大鼠血清中心肌酶LDH,CK-MB和AST含量降低,氧化指标GSH-Px,SOD含量升高,MDA含量降低,炎症因子MPO,IL-6和TNF-α降低,病理特征有明显改善。结论:维药黄花柳花总黄酮对心肌缺血再灌注损伤具有一定的保护作用。
OBJECTIVE: To investigate whether total flavonoids of Huanghua Liuhua can protect rats from myocardial ischemia-reperfusion injury in vivo and to explore its related mechanism. Methods: Ligation of left anterior descending coronary artery in rats for 30 min, resulting in ischemia and then reperfusion 2 h, real-time monitoring of ECG ST segment changes. Serum LDH, CK-MB, AST, GSH-PX, SOD, MDA, MPO, IL-6 and TNF-α were detected by blood sampling in abdominal aorta. Histopathological changes. Results: Compared with the model group, the levels of LDH, CK-MB and AST in serum were decreased, the contents of GSH-Px and SOD were increased, the content of MDA was decreased, the levels of MPO, IL-6 and TNF- α decreased, the pathological features have been significantly improved. Conclusion: The total flavonoids of Radix Huanghua Liuhua has a protective effect on myocardial ischemia-reperfusion injury.