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用神经细胞体外培养技术,在光、电镜下进行定量组织化学、免疫组化方法观察17—18天大鼠胚胎下丘脑神经元体外生长发育过程。结果显示:细胞培养3天时,神经元以2个突起为主,占81％;1周时,神经元以3个突起为主,占51％;2周时神经元以3～4个突起为主,分别占37％和31％。定量组化结果显示神经元SDH、AChE含量随培养时间延长而增多,在第3天分别为64.2和3.8,1周时分别为98.2和12.8,2周时分别为127.6和24.6。免疫组化结果显示培养神经元在第3天时,神经元特异烯醇酶(NSE)呈阳性反应。上述结果提示,体外培养胚胎大鼠下丘脑神经元在第3天基本发育成熟。本文还对体外培养神经元的特点进行了详细的描述。
In vitro culture of neural cells was used to observe the growth and development of hypothalamus neurons in vitro in 17-18 days by immunohistochemistry with light and electron microscopy. The results showed that neurons were mainly composed of two protuberances and accounted for 81% at the third day after culture. Three neurons were predominant in 51% of neurons at one week, and three or four protuberances Lord, respectively, 37% and 31%. Quantitative histochemical staining showed that the content of SDH and AChE in neurons increased with the prolongation of culture time, reaching 64.2 and 3.8 on the third day, respectively, 98.2 and 12.8 at the first week, and 127.6 and 24.6 at the second week respectively. Immunohistochemistry showed neuronal-specific enolase (NSE) positive neurons were cultured on day 3. The above results suggest that hypothalamic neurons of embryonic rat embryos basically mature on the third day. In this paper, the characteristics of neurons cultured in vitro are described in detail.