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目的:探讨晚期糖基化终产物受体(RAGE)基因多态性对中国汉族人群冠状动脉(冠脉)病变狭窄程度的影响。方法:应用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测109例冠脉轻中度狭窄(mild/moderate coronary stenosis,MCS)患者、134例重度狭窄(serious coronary stenosis,SCS)患者和170例健康对照者的RAGE基因3种多态性(-429T/C、1704G/T和G82S)基因型。结果:RAGE基因G82S多态性的S等位基因在SCS组的分布显著高于对照组(27.6%vs 13.2%,OR值为2.086,95%CI为1.494～2.914,P<0.001,Pcorr<0.003),而MCS组与对照组、MCS组与SCS组间差异无统计学意义(P>0.05,Pcorr=0.069);多变量Logistic回归分析提示与单倍体型T-Gly-T相比,单倍体型G-Ser-T发生冠脉SCS的危险显著增加(OR值为1.961,95%CI为1.150～3.343,P=0.013)。其余2种多态性(-429T/C和1704G/T)的基因型及等位基因频率分布在各组间差异无统计学意义(P>0.05)。结论:携带有RAGE基因G82S多态性的单倍体型发生冠脉SCS的危险性显著增加,提示该单倍体型与冠脉SCS的发生呈明显相关性。
Objective: To investigate the effect of advanced glycation end products receptor (RAGE) gene polymorphism on coronary artery (stenosis) coronary artery stenosis in Chinese Han population. Methods: One hundred and ninety-nine patients with mild-moderate coronary stenosis (MCS) and 134 severe stenosis (SCS) were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) ) Patients and 170 healthy controls were genotyped for the three polymorphisms (-429T / C, 1704G / T and G82S) of RAGE gene. Results: The distribution of S allele of G82S polymorphism in RAGE gene in SCS group was significantly higher than that in control group (27.6% vs 13.2%, OR = 2.086, 95% CI 1.494-2.914, P <0.001, Pcorr <0.003 ), But there was no significant difference between MCS group and control group, MCS group and SCS group (P> 0.05, Pcorr = 0.069). Multivariate Logistic regression analysis showed that haplotype T-Gly- There was a significant increase in the risk of developing coronary SCS for body size G-Ser-T (odds ratio 1.961, 95% CI 1.150-3.343, P = 0.013). The other two polymorphisms (-429T / C and 1704G / T) genotype and allele frequency distribution between the groups showed no significant difference (P> 0.05). CONCLUSION: The risk of coronary SCS haploidentally with G82S polymorphism of RAGE gene is significantly increased, suggesting that this haplotype has a significant correlation with the occurrence of coronary artery SCS.