论文部分内容阅读
采用9个ISSR(inter-simple sequence repeat)分子标记对云南南部魔芋属植物5个物种(花魔芋,疣柄魔芋,攸乐魔芋,勐海魔芋和屏边魔芋)105份材料进行了居群遗传多样性分析.通过PCR扩增,9个ISSR引物从5个物种105份样品中共获得95条条带,其中多态性条带占条带总数的100%.每个位点平均等位基因数2.00,有效等位基因数1.40,平均Nei’s基因多样性指数0.255 2,Shannon’s信息多样性指数为0.401 2.在这5个魔芋属植物物种中,花魔芋居群内遗传多样性水平相对较低.居群水平上的UPGMA聚类表明这5个物种可被分为明显的两大组,花魔芋为一组,其余4种构成一组,其中屏边魔芋与疣柄魔芋遗传距离最近.个体水平上的聚类则显示,花魔芋和疣柄魔芋的个体各自聚在一起,其余3种的个体较为分散.本研究结果还认为人工栽培利用的方式对魔芋属植物遗传多样性可能存在一定影响.
Nine inter-simple sequence repeat (ISSR) markers were used to detect the genetic diversity of five species of Amorphophallus in southern Yunnan Province (Amorphophallus konjac, Amorphophallus konjac, Amorphophallus konjac, Menghai konjac and Amorphophallus konjak) A total of 95 bands were obtained from 105 samples of 5 species by PCR amplification with nine ISSR primers, of which the polymorphic bands accounted for 100% of the total number of bands.The average number of alleles per locus 2.00, the number of effective alleles was 1.40, the average Nei’s gene diversity index was 0.255 2, and Shannon’s information diversity index was 0.401. Among the 5 species of Amorphophallus, the genetic diversity within Amorphophallus population was relatively low. UPGMA clustering on the population level indicated that the five species could be divided into two distinct groups, one was konjak, the other four were a group, and the genetic distance between konjac The clustering showed that individuals of Amorphophallus konjac and Amorphophallus konjac clustered together and the other 3 species were more dispersed.The results of this study also suggest that artificial cultivation may affect the genetic diversity of Amorphophallus.