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目的:利用生物信息学及分子生物学体外探讨剪切因子SC35对协同刺激分子B7-H3的表达调控机制。方法:通过生物信息方法筛选可能参与协同刺激分子B7-H3表达的调控蛋白,再通过定量PCR及RNA干扰实验验证剪切因子是否参与该分子的调控。结果:剪切因子SC35、SRP40、SF55可能参与B7-H3的表达调控,体外发现T细胞活化后B7-H3表达上调,SC35同时也表达上调;继而通过RNA干扰抑制SC35的表达,发现B7-H3的表达也同时降低。结论:剪切因子SC35可能参与了B7-H3的表达调控,对研究该分子的生物学功能奠定了基础。
OBJECTIVE: To investigate the regulatory mechanism of costimulatory molecule B7-H3 using shear stress factor SC35 in vitro in bioinformatics and molecular biology. METHODS: The regulatory proteins that may be involved in costimulatory molecules B7-H3 expression were screened by bioinformatics methods, and then the shear factors were involved in the regulation of this molecule by quantitative PCR and RNA interference. Results: The expression of B7-H3 was upregulated by SC35, SRP40 and SF55, and the expression of B7-H3 was up-regulated after T cell activation in vitro. The expression of SC35 was also up-regulated by RNA interference. The expression is also reduced at the same time. CONCLUSION: The cleavage factor SC35 may be involved in the regulation of B7-H3 expression, which lays the foundation for the study of the biological function of this molecule.