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本文建立了血清肌酸激酶BB同工酶(CK-BB)快速放射免疫测定(RIA)方法,灵敏度0.5ng/ml,测定范围0.5～100ng/ml,方法学鉴定符合RIA要求。本实验中正常组其值为1.3±1.8ng/ml。以CK-BB大于3.0ng/ml为阳性,急性心肌梗塞(AMI)组在心肌梗塞后8h男(n=22)、女(n=12)阳性率分别为86％和83％,24h均为100％。急性脑血管意外(CVA)组在脑血管意外后第1天男(n=24)、女(n=6)阳性率分别为8％和17％,第3天分别为79％和83％。方法特异性均为100％。
This article established a rapid radioimmunoassay (RIA) method of serum creatine kinase BB isoenzyme (CK-BB) with a sensitivity of 0.5 ng / ml and a determination range of 0.5-100 ng / ml. The methodological appraisal accords with RIA requirements. The normal group in this experiment its value 1.3 ± 1.8ng / ml. The positive rates of CK-BB> 3.0ng / ml in AMI group were 86% and 83% respectively at 8h after myocardial infarction (n = 22) 100%. The positive rates of male (n = 24) and female (n = 6) were 8% and 17% on the first day after cerebrovascular accident in the CVA group and 79% and 83% on the third day respectively. Method specificity was 100%.