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本文报道了用高效液相色谱法同时测定多种生物试样中的5-FU及其代谢产物FUR和FUdR。样品溶液的制备:当血清或胆汁中含有上述三种成份时,取样100μl,加0.5M磷酸盐缓冲溶液(pH 8.0)25μl和乙酸乙酯6.0ml振摇5分钟后离心分离,取有机相进行蒸发,残渣加流动相(HPLC)-n-C_5H_(14)(3:2)混合溶剂1ml,溶解后即可取样分析。当样品为组织时,取其200mg,加冷藏后的乙腈3ml和硅胶(60～80目)100mg,并拌匀,用乙
This paper reports the simultaneous determination of 5-FU and its metabolites FUR and FUdR in various biological samples by high performance liquid chromatography. Preparation of sample solution: When the serum or bile contains the above three components, take 100 μl of sample, add 25 μl of 0.5 M phosphate buffer solution (pH 8.0) and 6.0 ml of ethyl acetate and shake for 5 minutes, then centrifuge to take the organic phase Evaporation, the residue and mobile phase (HPLC) -n-C_5H_ (14) (3: 2) mixed solvent 1ml, after dissolution can be sampled and analyzed. When the sample is tissue, take 200mg, add 3ml of refrigerated acetonitrile and silica gel (60 ~ 80 mesh) 100mg, and mix well with B