NO参与真菌诱导子对红豆杉悬浮细胞中PAL活化和紫杉醇生物合成的促进作用

• 作 者: 徐茂军 董菊芳 朱睦元
• 机 构: 浙江大学生命科学学院植物生理学和生物化学国家重点实验室,杭州商学院食品、生物与环境工程学院,浙江大学生命科学学院植物生理学和生物化学国家重点实验室 杭州 310012 杭州商学院食品、生物与环境工程学
• 出 处: 科学通报
• 发表日期: 2004年07期
• 关键词: NO诱导子 信号分子 红豆杉悬浮细胞 紫杉醇

由桔青霉(Penicillium citrinum)细胞壁制备的诱导子可以诱发红豆杉悬浮细胞产生多种防卫反应,包括NO合成、PAL活化和紫杉醇合成加强.NO淬灭剂cPTIO和一氧化氮合酶(NOS)抑制剂PBITU可以阻断桔青霉诱导子对红豆杉悬浮细胞中PAL活化和紫杉醇合成的促进怍用.NO供体SNP单独处理也可以触发红豆杉细胞中PAL活化和紫杉醇合成加强.PBITU可以抑制桔青霉诱导子诱发红豆杉悬浮细胞中NO的增加.实验结果证实了在桔青霉诱导子处理下红豆杉悬浮细胞中NO合成与PAL活化和紫杉醇合成加强之间的因果关系,表明桔青霉诱导子诱导红豆杉悬浮细胞通过NOS产生NO,NO作为信号分子活化PAL并触发紫杉醇生物合成.

Elicitors made from the cell wall of Penicillium citrinum can induce a variety of defensive responses in Taxus suspension cells, including NO synthesis, PAL activation and paclitaxel synthesis enhancement.NO quenchers cPTIO and nitric oxide synthase (NOS) Inhibitor PBITU blocked the promotion of PAL activation and paclitaxel synthesis in Taxus suspension cells by the induction of Penicillium citrinum inducer.NP alone could also activate PAL activation and paclitaxel synthesis in Taxus cells.PBITU could inhibit Orange Penicillium elicitor induces an increase in NO in suspension cells of Taxus L. The experimental results confirm the causal relationship between NO synthesis and PAL activation and enhanced paclitaxel production in Taxus cuspidate suspension cells treated with the Penicillium citrinum inducer, Mold inducer induces Taxus cuspidate suspension cells to produce NO through NOS, with NO as a signaling molecule activating PAL and triggering paclitaxel biosynthesis.