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丙型肝炎病毒(HCV)的基因组提示HCV 是正链RNA 病毒。1989年,根据HCV cDNA 克隆36~6和376建立了检测肝、血浆或血清中HCV RNA 的敏感的半定量的cDNA/聚合酶链反应(cPCR)技术。本文报道用cPCR 法和放射免疫法(RIA)检测病人和实验黑猩猩的HCV RNA 和抗-C100-3抗体的结果。对象为15例慢性非甲非乙肝炎(NANBH),1例原因不明肝病,1例慢性HBV 感染的肝活检标本和血清,2例实验性HCV 感染的黑猩猩(771和910)的血浆和4份人类及4份黑猩猩的对照血清。从肝脏活检和血浆标本的RNA 提取液中合成
The genome of Hepatitis C virus (HCV) suggests that HCV is a positive-stranded RNA virus. In 1989, a sensitive semi-quantitative cDNA / polymerase chain reaction (cPCR) technique for the detection of HCV RNA in liver, plasma or serum was established based on HCV cDNA clones 36-6 and 376. This paper reports the results of cPCR and radioimmunoassay (RIA) assays of HCV RNA and anti-C100-3 antibodies in patients and experimental chimpanzees. The subjects were 15 patients with chronic non-A non-B hepatitis (NANBH), 1 with liver disease of unknown origin, 1 liver biopsy specimen with chronic HBV infection and serum, and 2 with experimental HCV-infected chimpanzees (771 and 910) and 4 Human and 4 chimpanzee control sera. Synthetic from RNA extracts of liver biopsies and plasma samples