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采用水培法研究了在富营养化条件下不同铵态氮与硝态氮比例(100∶0、75∶25、50∶50、25∶75、0∶100)对外来植物凤眼莲(Eichhornia crassipes)生长和生理响应的影响。结果表明,随着培养液中NO3-比例的增加,凤眼莲相对生长速率(RGR)升高,克隆分株数增多,根和叶片的NO3-浓度、硝酸还原酶(NR)活性升高。随NH4+比例的增加,尤其在NH4+∶NO3->1时,叶片NH4+浓度和GS活性变化不显著,而根的NH4+浓度、谷氨酰胺合成酶(GS)活性显著升高。研究结果表明,凤眼莲表现出对硝态氮的偏好性以及对NH4+极强的耐受能力。
Hydroponics was used to study the effects of Eichhornia (Eichhornia crassipes) on the eutrophication of Eichhornia crassipes (100: 0, 75:25, 50:50, 25:75, 0:100) crassipes) growth and physiological responses. The results showed that the relative growth rate (RGR) of Eichhornia crassipes increased with the increase of NO3 - ratio in the culture medium, and the number of clonal ramets increased. The NO3 - and nitrate reductase (NR) activities of roots and leaves increased. With the increase of NH4 + ratio, especially NH4 +: NO3-> 1, NH4 + concentration and GS activity did not change significantly, but NH4 + concentration and glutamine synthetase (GS) activity in roots were significantly increased. The results show that Eichhornia crassipes shows a preference for nitrate nitrogen and a strong tolerance to NH4 +.