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在模拟生理条件下,通过荧光光谱法研究染料木素(Ge)与牛血清蛋白(BSA)间相互作用,在水溶液中Ge能够有效地猝灭BSA的荧光发射。考查了溶液p H、水浴温度、水浴时间等参数对于Ge猝灭BSA的影响,并且通过StemVolmer方程计算了Ge与BSA之间的结合类型、结合位点数和结合常数等参数。结果表明,Ge猝灭BSA的类型为静态猝灭,Ge与BSA可形成1∶1型非共价复合物。通过同步荧光光谱法结合分子对接研究了Ge与BSA间的作用情况,结果表明,氢键作用与疏水作用是Ge与BSA形成复合物的主要驱动力,Ge通过改变BSA中Trp134残基微环境的疏水情况猝灭BSA的荧光发射。
Under simulated physiological conditions, the interaction between genistein (Ge) and bovine serum albumin (BSA) was studied by fluorescence spectroscopy. Ge could effectively quench BSA fluorescence emission in aqueous solution. The effect of pH, temperature of solution bath and bath time on the quenching of BSA by Ge was investigated. The binding type, number of binding sites and binding constants between Ge and BSA were calculated by StemVolmer equation. The results show that the type of quenching BSA for Ge is static quenching, and Ge and BSA can form non-covalent complexes of 1: 1 type. The interaction between Ge and BSA was studied by synchronous fluorescence spectroscopy and molecular docking. The results showed that hydrogen bonding and hydrophobic interaction are the main driving forces for the formation of complexes of Ge and BSA. Ge can be modified by changing the microenvironment of Trp134 residues in BSA Hydrophobic conditions quench the fluorescence emission of BSA.