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2008年从漳州采集到疑似由柑橘球座菌(Guignardia citricarpaKiely)引起的黑斑病症状的溪琯蜜柚(Citrus grandis(Linn.)Osbeck cv.guanxi-miyou)果实。使用柑橘球座菌(G.citricarpa)特异引物对该果实上的病斑进行PCR检测及序列分析。PCR检测结果表明从溪琯蜜柚果实病斑上扩增到约490bp的条带。序列分析结果表明,与已知的亚洲柑橘叶点霉(Phyllosticta citriasiana)、G.citricarpa和G.mangif-erae的相应序列同源性分别为99.8%、98.2%和94.0%。该序列含有部分的内转录间隔区1(ITS1)、5.8S核糖体RNA基因(5.8S rRNA)和部分的内转录间隔区2(ITS2)序列。结果表明,引起溪琯蜜柚果实病害的病原菌并不是G.citricarpa,而是一种新描述的病原菌——亚洲柑橘叶点霉(Phyllosticta citriasianaWulan-dari,Crous&Gruyter),该病原菌引起的病害称为柑橘褐斑病。
In 2008, the fruit of Citrus grandis (Linn.) Osbeck cv. Guanxi-miyou was collected from Zhangzhou and suspected to be caused by the black spot disease caused by Guignardia citricarpa Kiely. The spot on the fruit was subjected to PCR detection and sequence analysis using G.citricarpa specific primers. The results of PCR showed that about 490 bp band was amplified from the diseased platycoccus sinensis fruit. Sequence analysis showed that the homology with known Phyllosticta citriasiana, G.citricarpa and G.mangif-erae was 99.8%, 98.2% and 94.0%, respectively. This sequence contains part of the internal transcribed spacer 1 (ITS1), 5.8S ribosomal RNA gene (5.8S rRNA) and part of the internal transcribed spacer 2 (ITS2) sequence. The results showed that the pathogen causing the disease of the pomelo tree was not G.citricarpa, but a newly described pathogen called Phyllosticta citriasianaWulan-dari (Crous & Gruyter). The pathogen caused by this pathogen was called citrus Brown spot disease.