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获取大肠杆菌硫氧还蛋白基因（trxA），并进行序列测定，为今后研究其机理及应用创造条件．方法：用PCR法从大肠杆菌DNA中扩增trxA基因编码区，重组入pUC19质粒载体，用双脱氧法测定目的基因序列．结果：从大肠杆菌中成功地扩增到trxA基因，测出的序列与已知基因序列一致．结论：构建了trxA基因的重组克隆，为以后的深入研究奠定了基础．
The trxA gene of Escherichia coli was obtained and sequenced to create conditions for future study on its mechanism and application. Methods: The coding region of trxA gene was amplified by PCR from E. coli DNA and recombined into pUC19 plasmid vector. The target gene sequence was determined by dideoxy method. Results: The trxA gene was successfully amplified from E. coli and the sequence was consistent with the known gene sequence. Conclusion: The recombinant clones of trxA gene were constructed, which laid the foundation for further research.