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【目的】明确雷公藤Tripterygium wilfordii Hook.f.生物碱对粘虫Mythimna separata(Walker)神经系统的影响,为阐明其杀虫作用机制提供依据。【方法】采用载毒叶片法测定粘虫5龄幼虫经雷公藤总生物碱处理后体内乙酰胆碱酯酶(ACh E)、Na+,K+-ATPase、Ca2+,Mg2+-ATPase、谷丙转氨酶(GPT)和谷氨酸脱羧酶(GAD)等重要神经系统酶活性及乙酰胆碱(ACh)、谷氨酸(Glu)和γ-氨基丁酸(GABA)等神经递质的含量。【结果】雷公藤总生物碱处理对粘虫5龄幼虫ACh E无明显影响,麻醉期处理粘虫幼虫体内ACh相对含量与同期对照无显著差异。处理粘虫幼虫在轻度麻醉期、深度麻醉期和复苏期体内GABA和Glu含量显著升高,GABA含量分别升高了89.86%,49.28%和20.29%,Glu含量分别升高了24.55%,23.33%和8.13%。处理粘虫幼虫GPT活性明显受到抑制,而GAD活性无明显变化。处理明显抑制粘虫幼虫头部Na+,K+-ATPase和Ca2+,Mg2+-ATPase活性,但对中肠两种ATPase活性影响不大。【结论】研究结果有助于了解雷公藤生物碱对昆虫神经系统的影响,也为进一步阐明其作用靶标奠定了基础。
【Objective】 The objective of this study was to determine the effects of Tripterygium wilfordii Hook.f. alkaloids on the nervous system of Mythimna separata (Walker) and to provide a basis for elucidating its mechanism of insecticidal action. 【Method】 The acetylcholinesterase (AChE), Na +, K + -ATPase, Ca2 +, Mg2 + -ATPase and GPT in the 5th instar larvae of armyworm Glutamate decarboxylase (GAD) and other important nervous system enzyme activity and acetylcholine (ACh), glutamate (Glu) and γ-aminobutyric acid (GABA) and other neurotransmitters content. 【Result】 The total alkaloids of Tripterygium wilfordii had no significant effect on the ACh E of the 5th instar larvae of armyworm, and the relative content of ACh in the larvae of the armyworm during the anesthetic phase showed no significant difference compared with the same period. The contents of GABA and Glu in Moth armyworm larvae during mild, deep and recovery stages were significantly increased, the content of GABA increased by 89.86%, 49.28% and 20.29%, and the content of Glu increased by 24.55% and 23.33 % And 8.13%. The GPT activity of the armyworm larvae was significantly inhibited, while the GAD activity did not change significantly. The treatment significantly inhibited Na +, K + -ATPase and Ca2 +, Mg2 + -ATPase activity in the head of armyworm larvae, but had little effect on the activities of the two midgut ATPases. 【Conclusion】 The results are helpful to understand the influence of Tripterygium wilfordii alkaloids on the insect nervous system, and lay a foundation for further elucidating its target of action.