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本实验用的彭亨丝虫亚周期株的微丝蚴系用盛有肝素的注射器抽取微丝蚴阳性猫的静脉血取得的,以未感染的犬或猫血稀释至每40ml血含有300～350条微丝蚴,然后将其置于装置饲血膜的容器中,待血温平衡后给埃及伊蚊饲血。将吸血的蚊虫饲养于温度为26±1℃,相对温度为75～85%的蚊笼中。感染后9～12天将蚊虫压碎收集感染期幼虫经腹腔注射感染蒙古沙鼠。犬恶丝虫感染埃及伊蚊的方法同上。结果:5-氟尿嘧啶30mg/kg/天×4天腹腔注射沙鼠体内彭亨丝虫成虫无作用,在第
In this experiment, the microfilariae in the subfamily of Paenias filaria were obtained from the venous blood of positive cats of microfilariae using a syringe containing heparin and diluted to 300 to 350 per 40 ml of blood with an uninfected dog or cat blood Microfilaria, and then placed in a device feeding blood vessel, until the blood temperature equilibrated to Egypt Aedes blood. Blood-sucking mosquitoes are kept in a mosquito cage at a temperature of 26 ± 1 ° C and a relative humidity of 75-85%. Nine to twelve days after infection, mosquitoes were crushed and collected to infect gerbils infected by larvae. Dirofilaria infection Aedes aegypti method above. Results: 5-Fluorouracil 30mg / kg / day × 4 days in vivo injection of penicilliworm worms adult worms no effect in the first