论文部分内容阅读
目的 :从细胞分裂周期变化角度探讨生肌化瘀方促进创面修复及皮肤修复的机理。 方法 :采用体外培养创面肉芽组织成纤维细胞 ,并用乳鼠皮肤成纤维细胞作对照 ,分别加入用血清药理学方法制备的生肌方、化瘀方、生肌化瘀方大、小剂量药物血清。通过流式细胞仪检测成纤维细胞分裂周期的变化。 结果 :模型组细胞处于S期的比例低于正常组 (P <0 0 5 )。生肌方组细胞处于S期的比例明显高于正常组 (P <0 0 1)。化瘀方组细胞处于S期的比例明显低于正常组 (P <0 0 5 )。生肌化瘀方各剂量组细胞处于S期的比例均与正常组相比差异无显著性 (P >0 0 5 )。 结论 :生肌化瘀方通过调控创面肉芽组织成纤维细胞增殖周期的影响 ,使创面修复达到皮肤修复。
Objective : To explore the mechanism of Shengji Huayu Decoction in promoting wound repair and skin repair from the perspective of changes in cell division cycle. METHODS: The wound granulation tissue fibroblasts were cultured in vitro, and neonatal rat skin fibroblasts were used as controls. The serum, pharmacologically prepared Shengjifang, Huatanfang, Shengjihuahuafang large and small doses of drug serum were added respectively. . Changes in the fibroblast cell division cycle were examined by flow cytometry. Results: The proportion of cells in the S group was lower in the model group than in the normal group (P < 0.05). The proportion of cells in the S-phase group was significantly higher than that in the normal group (P <0 01). The proportion of cells in the S phase of the Huatanfang group was significantly lower than that of the normal group (P <0 05). There was no significant difference in the proportion of cells in the S phase of the Shengji Huatan Decoction group compared with the normal group (P > 0 05). Conclusion : The effect of Shengji Huayu Decoction can be achieved by regulating the proliferation cycle of fibroblasts in granulation tissue of wounds.