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通过检测高迁移率族蛋白B1(high mobility group protein B1,HMGB1)及Toll样受体2(toll like receptor 2,TLR2)在多发性硬化(multiple sclerosis,MS)患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达变化及血清中单核细胞趋化蛋白1(monocyte chemotactic protein 1,MCP-1)、IL-17分泌变化,初步探讨HMGB1及TLR2在MS发病中可能的免疫学作用。应用流式细胞术检测MS患者组、健康对照组人群PBMC中HMGB1及TLR2蛋白的相对表达并采用Pearson相关分析观察两者表达的相关性;应用ELISA法检测两组人群血清中MCP-1、IL-17分泌水平。MS组HMGB1、TLR2蛋白的相对表达及MCP-1、IL-17分泌水平较对照组明显上调(P<0.01),HMGB1蛋白与TLR2蛋白表达呈正相关(r=0.893,P<0.01)。HMGB1可能会通过其受体TLR2启动下游炎性信号传导通路,参与MS免疫损伤过程。
By detecting the high mobility group protein B1 (HMGB1) and toll like receptor 2 (TLR2) in patients with multiple sclerosis (MS), peripheral blood mononuclear cells (peripheral blood mononuclear cell (PBMC)) and the changes of monocyte chemotactic protein 1 (MCP-1) and IL-17 in serum, and to explore the possible immunological effects of HMGB1 and TLR2 in the pathogenesis of MS . Flow cytometry was used to detect the relative expression of HMGB1 and TLR2 protein in PBMC of MS patients and healthy controls. Pearson correlation analysis was used to observe the correlation between them. ELISA was used to detect the levels of MCP-1, IL -17 secretion level. The relative expression of HMGB1 and TLR2 protein and the secretion of MCP-1 and IL-17 in MS group were significantly higher than those in control group (P <0.01). There was a positive correlation between HMGB1 protein and TLR2 protein expression (r = 0.893, P <0.01). HMGB1 may initiate the downstream inflammatory signaling pathway through its receptor TLR2 and participate in the process of MS immune injury.