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目的:研究丹参素钠对大鼠局灶性脑缺血-再灌注损伤的治疗时间窗。方法:采用线栓法制备大鼠大脑中动脉闭塞所致局灶性脑缺血-再灌注损伤模型(MCAO),并于缺血后0、2、4、6 h腹腔注射给药,手术24 h后,观察丹参素钠对大鼠神经行为学评分、脑梗死体积、脑组织含水量及组织病理学的影响。结果:丹参素钠在缺血-再灌注后0~2 h内给药能显著改善MCAO大鼠的神经行为学评分,减轻脑水肿和减少脑梗死体积范围;缺血后4 h给药,疗效下降;缺血后6 h给药则无显著治疗作用。结论:丹参素钠对大鼠局灶性脑缺血-再灌注损伤的治疗时间窗为4h,以2 h内给药治疗效果最佳。
Objective: To study the therapeutic time window of sodium danshensu on focal cerebral ischemia-reperfusion injury in rats. Methods: The focal cerebral ischemia-reperfusion injury model (MCAO) induced by occlusion of the middle cerebral artery in rats was established by thread occlusion. The rats were injected intraperitoneally (0, 2, 4, 6 h) h, observe the effect of Danshensu sodium on neurobehavioral score, cerebral infarction volume, brain water content and histopathology. Results: Danshensu sodium administered within 0 ~ 2 h after ischemia-reperfusion could significantly improve the neurobehavioral scores, reduce brain edema and decrease the volume of cerebral infarction in MCAO rats. After 4 h of ischemia, Decreased; ischemic 6 h after administration there is no significant therapeutic effect. Conclusion: The time window of Danshensu sodium treatment for focal cerebral ischemia-reperfusion injury in rats is 4h, and the best treatment effect is given within 2h.