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目的探讨两歧双歧杆菌对菌群失衡大鼠类风湿关节炎(RA)的调整作用。方法首先利用抗生素头孢曲松钠灌胃的方法建立大鼠(SPF级雌性Wistar大鼠20只)肠道菌群失衡模型,在此基础上采用牛Ⅱ型胶原诱导方法建立大鼠RA(CIA)模型,然后分为模型组和两歧双歧治疗组,3周后,观察两组大鼠关节肿胀程度,血清中IgG、IL-1β、TNF-α、IL-6、IL-17、IL-4、IL-10的变化及血清中SOD、MDA和滑膜液中SOD的变化。结果与模型组相比,治疗组的关节肿胀评分有降低趋势;血清中的IgG(t=6.0114,P=0.0002)、IL-1β(t=6.6719,P=0.0001)、TNF-α(t=3.8461,P=0.004)和IL-17(t=4.6894,P=0.001)的含量明显降低,IL-6略有降低,IL-4和IL-10都有所升高;血清中的SOD活力有所升高,MDA含量有所降低,滑膜液中的SOD(t=-2.4793,P=0.038)活力明显升高。结论两歧双歧杆菌能够减缓炎症和降低氧化压力,从而出现减轻关节肿胀、延缓RA发展的趋势。
Objective To investigate the regulatory effect of Bifidobacterium bifidum on rheumatoid arthritis (RA) in rats with imbalanced flora. Methods The imbalance model of intestinal microflora in rats (SPF female Wistar rats) was established by intragastric administration of antibiotic ceftriaxone sodium. On the basis of this, the rat RA (CIA) Model, and then divided into model group and bifidobacterium bifido treatment group. After 3 weeks, the degree of joint swelling, the level of IgG, IL-1β, TNF-α, IL-6, IL- 4, IL-10 and serum SOD, MDA and synovial fluid SOD changes. Results Compared with the model group, the joint swelling score of the treatment group tended to decrease. The serum IgG (t = 6.0114, P = 0.0002), IL-1β (t = 6.6719, P = 0.0001) 3.8461, P = 0.004) and IL-17 (t = 4.6894, P = 0.001), while IL-6 and IL-10 decreased slightly The content of MDA decreased, and the activity of SOD in synovial fluid increased significantly (t = -2.4793, P = 0.038). Conclusions Bifidobacterium bifidum can reduce the inflammation and reduce the pressure of oxidation, thus reducing the swelling of joints and delaying the development of RA.