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目的探讨肺炎支原体DNA(MP DNA)检测结果对儿童肺炎的早期诊断价值。方法采用实时荧光定量PCR技术对本院957例门诊和住院患儿的呼吸道分泌物、痰液和血清的临床标本进行MP-DNA的定量检测。结果 MPDNA检测总阳性率为11.08%;男性和女性阳性率分别为9.12%和14.25%,女性明显高于男性,差异有统计学意义(χ2=5.512,P<0.05)。3岁~组阳性率最高,为15.04%(20/133),其次是6岁~14岁组阳性率为13.24%(47/355);夏季阳性率最高,为15.69%,其次是春季为11.89%。结论 3岁~组的儿童易感染肺炎支原体,且春、夏季节高发,加强MP-DNA的检测,有助于临床早期诊断和治疗,对预防肺外并发症有重要的临床意义。
Objective To investigate the early diagnosis of pneumonia in children with mycoplasma pneumoniae (MP DNA) test. Methods Quantitative detection of MP-DNA in 957 cases of outpatients and inpatients with respiratory secretions, sputum and serum from real-time fluorescence quantitative PCR was performed. Results The positive rate of MPDNA was 11.08%. The positive rates of male and female were 9.12% and 14.25%, respectively, which were significantly higher in female than in male (χ2 = 5.512, P <0.05). The positive rate was highest in the group of 3 years old (15.04%, 20/133), followed by that in the group of 6-14 years (13.24%, 47/355). The positive rate was 15.69% in summer, followed by 11.89 in spring %. Conclusion The 3-year-old children are susceptible to Mycoplasma pneumoniae infection. In addition, the detection of MP-DNA may be helpful for the early diagnosis and treatment of MP. It is of great clinical significance to prevent extra-pulmonary complications.