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利用电镜负染技术检测自然海区养殖和人工感染试验的栉孔扇贝组织提取液,分别发现病毒和类立克次氏体(RLO)两种病原体。动态检测两类病原体感染状况与整个养殖期间栉孔扇贝发病死亡情况,结果表明,养殖期间病毒感染率在80%～100%之间,最大感染强度出现在栉孔扇贝大规模死亡的7、8月份。人工感染病毒的栉孔扇贝,其病毒感染率和感染强度与养殖栉孔扇贝大规模死亡时期相当。而两种情况下栉孔扇贝类立克次氏体的感染率和感染强度均未见明显变化。综合分析表明,病毒可能是造成栉孔扇贝大规模死亡的直接病原体。
Electron microscopy negative staining technique was used to detect the tissue culture extracts of Chlamys farreri and the rickettsia-like rickettsia (RICK) pathogen isolated from the culture and artificial infection test in the natural sea area. The results showed that the virus infection rate was between 80% and 100% during the breeding period, and the maximum infection intensity occurred in the large-scale death of Chlamys farreri 7 and 8 month. Artificially infected chlamys farreri virus, the virus infection rate and infection intensity and breeding scallop chlamydia mortality period is quite. In both cases, no significant changes were found in the infection rates and infection intensities of rickettsia chlamys. Comprehensive analysis showed that the virus may be the direct cause of large-scale death of Zhikong scallops.