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磷酸肌酸激酶(creatine phosphokina-se,CPK)及其同功酶活性测定,对于心肌、骨骼肌和中枢神经系统损伤的诊断有一定意义。近年来国外对CPK同功酶活性测定及其在新生儿临床的应用进行了较多研究,引起人们高度重视。CPK 概况CPK又称三磷酸腺苷:磷酸肌酸转移酶,催化肌酸与三磷酸腺苷作用生成磷酸肌酸和三磷酸腺苷的可逆反应,酶分子量81,000。1964年Burger证明CPK分子结构由M和B两个亚单位组成,因而有三种同功酶形式,即BB型(脑型,CPK-Ⅰ型)、MB型(心型,CPK-Ⅱ型)和MM型(肌型,CPK-Ⅲ型)。CPK为一种器官特异性酶,正常组织中MM在骨骼
Determination of creatine phosphokinase (CPK) and its isoenzyme activity has some significance for the diagnosis of myocardial, skeletal and central nervous system injuries. In recent years, foreign CPK isoenzyme activity determination and its clinical application in neonatal more research has aroused people’s attention. CPK Overview CPK, also known as adenosine triphosphate: creatine phosphokinase, catalytic creatine and adenosine triphosphate to produce phosphocreatine and adenosine triphosphate reversible reaction, the molecular weight of 81,000 .1964 Burger proves CPK molecular structure consists of M and B two subunits, Therefore, there are three kinds of isozymes: BB type (brain type, CPK-Ⅰ type), MB type (heart type, CPK-Ⅱ type) and MM type (muscle type, CPK-Ⅲ type). CPK is an organ-specific enzyme, with MM in the bone in normal tissues