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目的:研究前列通瘀提取液对体外培养前列腺基质平滑肌细胞增殖与凋亡的作用。方法:分别以1、2、10、50、100×10-5g/L 5种不同浓度前列通瘀提取液作用体外培养前列腺基质平滑肌细胞,采用MTT和TUNEL法分别测定抗增殖指数和凋亡指数。结果:5种不同浓度前列通瘀提取液作用48 h后抗增殖指数分别为50.61%、53.52%、56.92%、65.53%、72.94%,抗增殖效果随着浓度增大而增大。100×10-5g/L前列通瘀提取液作用24、48、72 h后凋亡指数与对照组比较差异无显著性。结论:(1~100)×10-5g/L前列通瘀提取液体外作用前列腺基质平滑肌细胞具有显著的抗增殖作用。
Objective: To study the effects of Qianlie Tongqiao extract on the proliferation and apoptosis of prostate stromal smooth muscle cells in vitro. METHODS: Prostate-derived smooth muscle cells were cultured in vitro with 1, 2, 10, 50, and 100×10-5 g/L of five different concentrations of forsythia panax extracts. MTT and TUNEL methods were used to measure the anti-proliferative index and apoptosis index, respectively. . Results: The antiproliferative index of 5 different concentrations of Qianlietongyu extract was 50.61%, 53.52%, 56.92%, 65.53% and 72.94% after 48 hours, and the anti-proliferation effect increased with the increase of the concentration. There was no significant difference in the apoptotic index between the control group and the apoptotic index at 24, 48, and 72 h after 100×10-5 g/L Qianlietongxiong extract. Conclusion: (1~100)×10-5g/L Qianlietongtong has significant anti-proliferative effect on the proliferation of prostate smooth muscle cells.