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采用机械粉碎、高温、酸碱处理等方法对稻草秸秆进行预处理,以烟曲霉为实验菌株,研究预处理方法对菌株产纤维素酶的影响。结果表明,取机械粉碎后的稻草(30~120目)进行121℃高压蒸汽处理20min(即灭菌处理),有利于菌株的生长与纤维素酶的产生;与未粉碎的稻草秸秆相比,烟曲霉羧甲基纤维素钠(CMC)酶、微晶纤维素酶、β-葡萄糖苷酶和滤纸(FPA)酶的活力分别提高了63.2%、164.0%、10.2%和14.1%。而采用不同种类、不同浓度的酸碱常温处理稻草秸秆4d或100℃高温处理30min,纤维素酶活力均出现了不同程度的下降。
Straw straw was pretreated by mechanical smashing, high temperature, acid-base treatment and so on. Aspergillus fumigatus was used as experimental strain to study the effect of pretreatment on cellulase production. The results showed that mechanical crushing of straw (30-120 mesh) was carried out at 121 ℃ for 20 min under high pressure steaming (ie, sterilization treatment), which was conducive to the growth of the strain and the production of cellulase. Compared with the non-pulverized straw, The activities of Aspergillus fumigatus carboxymethyl cellulose (CMC) enzyme, microcrystalline cellulose, β-glucosidase and filter paper (FPA) enzyme increased by 63.2%, 164.0%, 10.2% and 14.1%, respectively. However, using different kinds and concentrations of acid-base at normal temperature for 4 days or 100 ℃ for 30 minutes, the cellulase activity of rice straw decreased with different degrees.