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为了更好地应用SSR分子标记技术于濒危植物海南龙血树保护遗传学研究,本研究通过单因子试验与正交试验方法,优化建立海南龙血树SSR-PCR反应体系,并对优化后体系的稳定性进行了检测.研究结果表明,优化后的海南龙血树15 μL SSR-PCR反应体系为:1.5 μL 10 ×PCR buffer,Mg2+浓度为2.0 mmol/L,dNTPs浓度为100 μmol/L,Taq酶1.0U,引物0.4 μmol/L和DNA模板5 ng.经验证,该反应条件可用于海南龙血树遗传多样性和遗传结构分析.“,”In order to better use the SSR molecular marker in the conservation genetics study of Dracaena cambodiana,this study used single factor test and orthogonal test method to optimize SSR-PCR reaction system in D.cambodiana,and the stability of the optimized system was tested.The results showed that the optimized 15 μL SSR-PCR reaction system ofD.cambodiana included 10×buffer 1.5 μL,2.0 mmol/L MgC12,100 μmol/L dNTPs,Toq DNA polymerase 1.0 U,Primer 0.4 μmol/L and DNA template 5 ng.After verification,the optimized SSR-PCR reaction system was suitable for the analysis of genetic diversity and genetic structure ofD.cambodiana population.