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1989年美国Chiron公司Choo等首次将现代分子生物学技术直接应用于寻找未知病毒,并率先获得血源性NANB—VCDNA分子克隆。随后,日本等许多学者以Chiron公司公布的碱基序列为基础,采用特异性引物的逆转录反应,PCR及序列分析,或参照Chiron公司随机引物分子克隆和免疫筛选法。立即着手对HCV的全基因序列及其变异株序列进行研究。其发展速度十分迅速,1990年就阐明了数株HCV的部分片段或全基因序列得以公布。1 HCV的结构和功能 HCV为单股正链RNA,全长约9.5kb,由5’—NC及中间编码区,3’—NC三部分构成,迄今发现最长的5’—NC区序列为341核苷酸(nt),能形成
In 1989, for example, Choo Company of Chiron, USA, applied the modern molecular biology technology directly to search for unknown viruses and took the lead in obtaining the hemacytic NANB-VCDNA molecular clone. Subsequently, many scholars in Japan, based on the nucleotide sequences published by Chiron Corporation, used specific primers for reverse transcription reaction, PCR and sequence analysis, or Chiron’s random primer molecular cloning and immunoscreening methods. Immediately proceed to study the HCV whole genome sequence and its variant sequences. Its development speed is very rapid, in 1990 it clarified that several strains of HCV part of the whole genome sequence or published. 1 The structure and function of HCV HCV single strand positive strand RNA, a total length of about 9.5kb, from the 5’-NC and the middle coding region, 3’-NC three parts, so far found that the longest 5’-NC region sequence is 341 nucleotides (nt), can form