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采用更加敏感的半重叠式TCRγ特异性引物的PCR方法，对28例ALL初治、CR及BMT患儿进行检测，并用消化煮沸及酚抽提法分别对所有骨髓标本进行DNA提取，将PCR结果进行比较。结果表明：消化煮沸法用于微量标本的DNA提取优于酚抽提法，28例ALL患儿中16例检出TCRγ特异性条带，MRD组18例，阳性检出12例，其中免疫分型标记为B细胞型的4例，占25％，免疫标记为T细胞型者全部出现TCRγ阳性条带。并对该引物的语系特异性及双克隆重排问题进行了理论探讨。
Twenty-eight children with ALL, CR, and BMT were detected by PCR with more sensitive semi-overlapping TCRγ-specific primers. DNA was extracted from all bone marrow samples by digestive boiling and phenol extraction. PCR results were obtained. Compare. The results showed that the DNA extraction method for the microbiological specimens was better than the phenol extraction method in the digestive boiling method. TCRγ-specific bands were detected in 16 of the 28 children with ALL, 18 of them were in the MRD group, and 12 were positive in the MRD group. Four of the B-cells were type-marked, accounting for 25%. All T-lymphocytes with immunolabeling were TCR-gamma-positive. The theoretical study of the language-specificity and double-clone rearrangement of the primer was discussed.