己酮可可碱保护内毒素诱导急性肺损伤的实验研究

来源 :中国急救医学 | 被引量 : 0次 | 上传用户:qdjmyuzhou
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目的 探讨急性肺损伤的治疗方法。方法 建立大鼠急性肺损伤模型 ,随机分成 3组 ,筛选药物己酮可可碱治疗 ,检测支气管肺泡灌洗液中性白细胞及巨噬细胞比例和肺通透指数 ,肺组织湿重 /干重 ,肺血管通透性 ,一氧化氮 (NO)、诱生性一氧化氮合成酶 (iNOS)、内皮素 (ET)测定和肺组织大体、病理观察。结果 治疗组与内毒素组比较 ,中性白细胞比例下降 ,肺通透指数减低 ,肺血管通透性下降 ,NO、iNOS、ET明显下降 ,肺组织大体明显改善 ,病理观察损伤肺组织修复 ;与生理盐水组差异不明显。结论 己酮可可碱能有效防治由内毒素诱导的急性肺损伤 Objective To investigate the treatment of acute lung injury. Methods Acute lung injury model was established in rats. The rats were randomly divided into 3 groups. Pentoxifylline was screened. The ratio of neutrophils and macrophages in bronchoalveolar lavage fluid, the index of pulmonary permeability, the wet weight / dry weight of lung tissue, Pulmonary vascular permeability, nitric oxide (NO), inducible nitric oxide synthase (iNOS), endothelin (ET) and lung tissue were observed. Results Compared with the endotoxin group, the ratio of neutrophils decreased, the index of pulmonary permeability decreased, the permeability of pulmonary vessels decreased, NO, iNOS and ET decreased significantly, and the lung tissues were generally improved obviously. There was no obvious difference in saline group. Conclusion Pentoxifylline can effectively prevent and treat acute lung injury induced by endotoxin
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